Analytical Biochemistry 2013-03-01

Single-step cloning of a hybridoma producing a monoclonal antibody against a target protein.

Hidefumi Orii, Takeshi Yamaguchi, Kenji Watanabe

Index: Anal. Biochem. 434(1) , 52-3, (2013)

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Abstract

We have developed a simple and time-saving method to identify hybridoma clones producing an antibody against a target protein among a large number of hybridomas in a single step. This method is very useful as the primary screening tool for hybridomas producing monoclonal antibodies if several micrograms of the target protein is available.Copyright © 2012 Elsevier Inc. All rights reserved.