Analytical Biochemistry 2010-02-01

Coating of nitrocellulose for colorimetric DNA microarrays.

Marina Cretich, Valentina Sedini, Francesco Damin, Maria Pelliccia, Laura Sola, Marcella Chiari

Index: Anal. Biochem. 397 , 84-88, (2010)

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Abstract

We report on the modification of a nitrocellulose film with copoly(DMA-NAS-MAPS), a tercopolymer based on N,N-dimethylacrylamide (DMA), N-acryloyloxysuccinimide (NAS), and 3-(trimethoxysilyl)propyl-methacrylate (MAPS). The chains of this polymer, interacting with nitrocellulose fibers, introduce active ester functionalities that promote the covalent binding of short oligonucleotide fragments to the nitrocellulose thin film. Using colorimetric detection, naked eye visible DNA microarrays are developed for easy identification of foodborne pathogens. The fast and robust procedure of nitrocellulose functionalization opens the opportunity to implement this material in disposable analytical microdevices that do not require sophisticated readout systems.Copyright 2009 Elsevier Inc. All rights reserved.