Biological Chemistry Hoppe-Seyler 1990-09-01

Synthesis of a water-soluble protein cross-linking reagent.

U M Kremer

Index: Biol. Chem. Hoppe-Seyler 371(9) , 861-4, (1990)

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Abstract

2,4-Difluoro-5-nitrobenzenesulfonic acid has been synthesized by the sulfonation of 2,4-difluoronitrobenzene, and precipitated with KCl as the potassium sulfonate. The structure was confirmed by chemical and spectroscopic methods (IR, 1H-NMR, 13C-NMR, 19F-NMR, UV, MS and ultimate organic analysis). Lysozyme was cross-linked with the potassium sulfonate and with 1,5-difluoro-2,4-dinitrobenzene. The products were analysed by SDS-PAGE and compared. The cross-linking conditions were optimized.