Genome Biology 2008-01-01

Gene expression-based screening for inhibitors of PDGFR signaling.

Alena A Antipova, Brent R Stockwell, Todd R Golub

Index: Genome Biol. 9 , R47, (2008)

Full Text: HTML

Abstract

Here we describe a proof-of-concept experiment designed to explore the possibility of using gene expression-based high-throughput screening (GE-HTS) to find inhibitors of a signaling cascade, using platelet derived growth factor receptor (PDGFR) signaling as the example. The previously unrecognized ability of aurintricarboxylic acid to inhibit PDGFR signaling, discovered through a screen of 1,739 compounds, demonstrates the feasibility and generalizability of GE-HTS for the discovery of small molecule modulators of any signaling pathway of interest.