Drug Development and Industrial Pharmacy 1998-11-01

Particle size determination of a flocculated suspension using a light-scattering particle size analyzer.

A Bommireddi, L C Li, D Stephens, D Robinson, E Ginsburg

Index: Drug Dev. Ind. Pharm. 24(11) , 1089-93, (1998)

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Abstract

Microscopy is a useful and direct method for measuring the particle size of a suspension because, in addition to the particle size and size distribution, it provides visual detection of the shape and state of aggregation of the particles in the suspension. However, this method suffers from the shortcomings of being tedious and time consuming. In this study, a light-scattering particle size analyzer was used to determine the particle size and size distribution of a flocculated suspension. The sonication of the sample prior to and during measurement was found to be critical in ensuring that data are representative of the size distribution of the primary particles of the suspension. The light-scattering results were further confirmed by data generated using a polarized light microscope equipped with an image analyzer.