Bioorganic & Medicinal Chemistry Letters 2001-11-19

Visualizing antibody-catalyzed retro-aldol-retro-Michael reactions.

F Tanaka, L Kerwin, D Kubitz, R A Lerner, C F Barbas

Index: Bioorg. Med. Chem. Lett. 11(22) , 2983-6, (2001)

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Abstract

We developed a visible detection system for antibody-catalyzed retro-aldol-retro-Michael reactions. Aldolase antibody 38C2 catalyzed the reaction of substrate 1 to provide 6-bromo-2-napthol that forms a visible colored azo dye with diazonium salts. This system has potential for the screening of novel catalysts.