Life Sciences 1995-01-01

Cannabinoid photolabelling of a G-protein gamma-subunit in mouse peritoneal cells.

S H Burstein, M Debatis, A Subramanian

Index: Life Sci. 56(23-24) , 1991-8, (1995)

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Abstract

Binding and photoactivation of a cannabinoid-derived ligand to intact mouse peritoneal cells has resulted in the labelling of a G-protein gamma-subunit. The assignment of structure is based on the product's physical characteristics and its ability to react with a polyclonal antiserum raised against the partial amino acid sequence for a gamma-subunit expressed in spleen cells. The binding characteristics of the ligand to the cells suggests that this gamma-subunit, and its associated alpha and beta subunits, are located in close proximity to one of the transmembrane cannabinoid receptors. Our findings further suggest a possible experimental approach to identifying receptor-G-protein complexes in intact cells.