Protein Expression and Purification 1992-10-01

Isolation of amine oxidase from bovine plasma by a two-step procedure.

F Vianello, M L Di Paolo, L Zennaro, R Stevanato, A Rigo

Index: Protein Expr. Purif. 3(5) , 362-7, (1992)

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Abstract

A novel method for isolation of amine oxidase from bovine plasma is reported; it involves a two-step procedure, namely ammonium sulfate fractionation and affinity chromatography with elution by aniline, which is a competitive inhibitor of the enzyme. A homogeneous enzyme, characterized by a specific activity of 0.44 U/mg, was obtained with a yield higher than 50%.