Gene 1994-04-08

The aminopeptidase N-encoding pepN gene of Streptomyces lividans 66.

M J Butler, J S Aphale, C Binnie, M A DiZonno, P Krygsman, G A Soltes, E Walczyk, L T Malek

Index: Gene 141(1) , 115-9, (1994)

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Abstract

The gene (pepN) encoding an aminopeptidase N (PepN) has been cloned from Streptomyces lividans. This was done using either leucine-beta-naphthylamide or arginine-beta-naphthylamide in a liquid overlayer on colonies growing on agar medium to screen for overproduction of the ability to hydrolyse the substrates. The nucleotide sequence of pepN was determined and shown to encode a 95-kDa protein, which displayed significant homology to PepN proteins from other organisms. Analysis of the overproduced proteinase confirmed that this protein was located intracellularly as a monomeric active species. PepN is a metallo-exopeptidase cleaving next to Leu, Arg and Lys in peptide-bond-containing substrates.