Journal of enzyme inhibition 1998-08-01

Inhibition of succinic semialdehyde dehydrogenase by N-formylglycine.

S Kumar, N S Punekar

Index: J. Enzym. Inhib. 13(5) , 369-76, (1998)

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Abstract

N-formylglycine was developed as a dead-end inhibitor of the succinic semialdehyde dehydrogenase reaction. At 4 mM, it inhibited Aspergillus niger succinic semialdehyde dehydrogenase by 40%. N-formylglycine is a reversible, complete inhibitor; the inhibition is competitive with succinic semialdehyde and uncompetitive with respect to NAD+ and the Ki values are 4.9 and 10.4 mM respectively. Potato succinic semialdehyde dehydrogenase is also inhibited by N-formylglycine to a similar extent, the nature of the inhibition being identical to that observed with the A. niger enzyme.