Revista latinoamericana de microbiologia 2002-01-01

Determination of the profile of fatty acids of 4 species of Shigella spp by chromatography of gases.

E Robles Valderrama, M D Hurtado Bocanegra, M E González Arreaga, P Ramírez García, A Durán Díaz, R Ayala Patiño, M E Martínez Pérez

Index: Rev. Latinoam. Microbiol. 44 , 69-74, (2002)

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Abstract

Shigella boydii, Shigella flexneri, Shigella dysenteriae and Shigella sonnei were identified using gas chromatography instead of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 192 samples were analyzed both reference strains (ATCC 8700, INDRE B2188, B2194 and B2199) and environmental isolates. 12 fatty acids were included in the profiles from which 3-hydroxytetradecanoic acid (peak 12), trans 9-octadecanoic acid (peak 22), heptadecanoic acid (peak 18) and octadecanoic acid (peak 23), were the most important for the differentiation of the species analyzed.