PNAS 1987-07-01

Parathyroid hormone-related protein purified from a human lung cancer cell line.

J M Moseley, M Kubota, H Diefenbach-Jagger, R E Wettenhall, B E Kemp, L J Suva, C P Rodda, P R Ebeling, P J Hudson, J D Zajac

Index: Proc. Natl. Acad. Sci. U. S. A. 84 , 5048, (1987)

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Abstract

A protein with biological activities similar to parathyroid hormone (PTH) has been purified from serum-free culture medium obtained from a human lung cancer cell line (BEN). A major protein band of 18 kDa was obtained on NaDodSO4/polyacrylamide gels, with faint bands at 35 kDa and 67 kDa. Biological activity was associated only with the 18-kDa band. Amino acid sequence analysis of the material purified by HPLC revealed that 8 of the 16 residues were identical with those of human PTH. Antibody raised to a corresponding synthetic peptide recognized the PTH-related material but showed less than 1% cross-reactivity with human PTH amino-terminal peptides. BEN cells contained PTH DNA, but not PTH messenger RNA, indicating involvement of another gene. The purified PTH-related protein had a specific biological activity approximately equal to 6 times greater than that of bovine PTH(1-34). The PTH-related protein may have a role in the syndrome of humoral hypercalcemia of malignancy.