Yao xue xue bao = Acta pharmaceutica Sinica 2004-10-01

Influence of N,N'-diacetyl-L-cystine on D-galactosamine/lipopolysaccharide induced immunological liver failure in mice.

Pei Wang, Xing-chang Ren, Jin Yu, Yi Lin, Xi-ming Wu

Index: Yao Xue Xue Bao 39(10) , 782-6, (2004)

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Abstract

To study the therapeutic effects of N,N'-diacetyl-L-cystine (DiNAC) on immunological liver failure.Serum ALT, AST and T cell subsets in peripheral blood of the experimental animals during the trial period were analyzed by an automatic serum analyzer and a flow cytometer, respectively. The sectioned liver specimens were examined under a light microscope. And 24 h after the injection of Gal/LPS, the survival rate of rats was calculated.DiNAC (50, 200, 800 mg x kg(-1), i.p.) suppressed the elevation of serum levels of ALT and AST, markedly enhanced proliferation and differentiation of T cell subsets (CD4+, CD8+ and Th1, Th2), and improved all the histopathological features. In mice of fulminant hepatic failure (FHF), the survival time significantly prolonged and the survival rate increased 24 h after i.p. DiNAC. These effects were obviously dose-dependent.DiNAC on mice with FHF has an inhibitory action which is related to immune mechanism.