FEBS Letters 1998-08-21

Corpeptins, new bioactive lipodepsipeptides from cultures of Pseudomonas corrugata.

M C Emanuele, A Scaloni, P Lavermicocca, N S Jacobellis, L Camoni, D Di Giorgio, P Pucci, M Paci, A Segre, A Ballio

Index: FEBS Lett. 433(3) , 317-20, (1998)

Full Text: HTML

Abstract

The structure of the corpeptins, bioactive lipodepsipeptides produced in culture by Pseudomonas corrugata, the causal agent of tomato pith necrosis, has been determined. The combined use of FAB-mass spectrometry, NMR spectroscopy and chemical procedures has allowed us to assign the following primary structure to the peptide moiety: Dhb-Pro-Ala-Ala-Ala-Val-Val-Dhb-Hse-Val-alle-Dhp-Ala-Ala-Ala-Val-D hb-aThr-Ala-Dab-Ser-Ile with the terminal carboxy group closing a macrocyclic ring on the hydroxy group of the allo-threonine residue. The N-terminus is in turn acylated by 3-hydroxydecanoate in corpeptin A and by cis-3-hydroxy-5-dodecenoate in corpeptin B. Some preliminary data on the biological activity of corpeptins are included.