Applied and Environmental Microbiology 2004-07-01

Thioesterase II of Escherichia coli plays an important role in 3-hydroxydecanoic acid production.

Zhong Zheng, Qiang Gong, Tao Liu, Ying Deng, Jin-Chun Chen, Guo-Qiang Chen

Index: Appl. Environ. Microbiol. 70(7) , 3807-13, (2004)

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Abstract

3-Hydroxydecanoic acid (3HD) was produced in Escherichia coli by mobilizing (R)-3-hydroxydecanoyl-acyl carrier protein-coenzyme A transacylase (PhaG, encoded by the phaG gene). By employing an isogenic tesB (encoding thioesterase II)-negative knockout E. coli strain, CH01, it was found that the expressions of tesB and phaG can up-regulate each other. In addition, 3HD was synthesized from glucose or fructose by recombinant E. coli harboring phaG and tesB. This study supports the hypothesis that the physiological role of thioesterase II in E. coli is to prevent the abnormal accumulation of intracellular acyl-coenzyme A.