Bioorganic & Medicinal Chemistry Letters 1999-01-04

Cleavage of L-leucine-containing dipeptides by Clostridium butyricum.

N Khelifa, M Brik, A C Tessedre, H De Rocquigny, B P Roques, J Courtieu, A Rimbault

Index: Bioorg. Med. Chem. Lett. 9 , 109-112, (1999)

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Abstract

The ability of Clostridium butyricum cultures to hydrolyze three L-leucine-containing dipeptides (Leu-Leu, Leu-Gly and Gly-Leu) in a synthetic minimal medium is demonstrated by using gas chromatography coupled with mass spectrometry. The 13C nuclear magnetic resonance and a labeled dipeptide L-[1-13C]Leu-Gly were used to confirm this activity.