Protein Science 2011-12-01

A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin.

Yu Wang, Zhipu Luo, Xiaoli Shi, Huan Wang, Lei Nie, Mingdong Huang

Index: Protein Sci. 20(12) , 2095-101, (2011)

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Abstract

11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.Copyright © 2011 The Protein Society.