Stabilizing and destabilizing effects of phenylalanine→ F5-phenylalanine mutations on the folding of a small protein

…, EB Hadley, S Mecozzi, SH Gellman

Index: Woll, Matthew G.; Hadley, Erik B.; Mecozzi, Sandro; Gellman, Samuel H. Journal of the American Chemical Society, 2006 , vol. 128, # 50 p. 15932 - 15933

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Citation Number: 83

Abstract

We report a systematic evaluation of phenylalanine-to-pentafluorophenylalanine (Phe→ F5- Phe) mutants for the 35-residue chicken villin headpiece subdomain (c-VHP), the hydrophobic core of which features a cluster of three Phe side chains (residues 6, 10, and 17). Phe→ F5-Phe mutations are interesting because aryl-perfluoroaryl interactions of optimal geometry are intrinsically more favorable than aryl-aryl interactions and because ...