Bioorganic & Medicinal Chemistry Letters 2010-02-01

Synthesis and application of a photoaffinity analog of dehydroepiandrosterone (DHEA).

Horacio F Olivo, Nury Perez-Hernandez, Dongmin Liu, Mary Iruthayanathan, Brianne O'Leary, Laurie L Homan, Joseph S Dillon

Index: Bioorg. Med. Chem. Lett. 20 , 1153-1155, (2010)

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Abstract

We have synthesized an analog of dehydroepiandrosterone (DHEA, 1) containing both a benzophenone (BP) and a biotin (Bt) group (DHEA-BP-Bt, 8). Compound 8 was prepared by functionalization on C-17 of 1. Biocytin was reacted with 4-benzoylbenzoic acid and the product was condensed with 1 containing a diamine-hexane linker. We detected specific protein bands of approximately 55, 80, and 150 kDa by SDS-PAGE analysis of vascular endothelial cell plasma membranes which had been photoirradiated in the presence of 8.Copyright (c) 2009 Elsevier Ltd. All rights reserved.