Bioscience, Biotechnology, and Biochemistry 2002-04-01

Synthesis of (R)-1,3-butanediol by enantioselective oxidation using whole recombinant Escherichia coli cells expressing (S)-specific secondary alcohol dehydrogenase.

Hiroaki Yamamoto, Akinobu Matsuyama, Yoshinori Kobayashi

Index: Biosci. Biotechnol. Biochem. 66(4) , 925-7, (2002)

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Abstract

The synthesis of (R)-1,3-butanediol (BDO) from its racemate was studied using whole cells of recombinant Escherichia coli expressing an (S)-specific secondary alcohol dehydrogenase (CpSADH) from Candida parapsilosis by enantioselective oxidation. Under the optimized conditions, the yield of (R)-1,3-BDO reached 72.6 g/l, with a molar recovery yield of 48.4% from a racemate of 15% and an optical purity of 95% ee.