Environmental Health Perspectives 1994-05-01

Regulation of glucose transport in the NIH 3T3 L1 preadipocyte cell line by TCDD.

H Olsen, E Enan, F Matsumura

Index: Environ. Health Perspect. 102(5) , 454-8, (1994)

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Abstract

This study examined the changes in cellular glucose uptake induced by 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) as measured by quantification of intracellular radioactivity in the NIH 3T3 L1 preadipocyte cell line after a 30-minute incubation with the non-metabolizable radioactive analogue of glucose, 3-O-methyl-D-[1-3H] glucose. Treatment of differentiated NIH 3T3 L1 cells with TCDD produced a time- and dose-dependent decrease in the cellular uptake of glucose. Treatment of cells for 3 hr with 10(-8) M TCDD significantly reduced glucose uptake to about 10% of control values (p