A turn-on fluorescent indicator for citrate with micromolar sensitivity.
M Isabel Burguete, Francisco Galindo, Santiago V Luis, Laura Vigara
Index: Dalton Trans. (36) , 4027-33, (2007)
Full Text: HTML
Abstract
A turn-on fluorescent indicator for citric acid (citrate) has been developed, displaying high emission enhancement (+1500%) and low interference by other carboxylates. The sensor is based on the non-emissive copper(II) complex of a fluorescent amino amide, which, upon addition of citrate decomplexates to yield the emissive ligand. The detection limit estimated for this new chemosensing system is about 0.5 microM. This novel approach to the analysis of citrate constitutes an alternative ca. 10(2)-10(3) times more sensitive than the standard method based on the enzyme citrate lyase.
Related Compounds
| Structure | Name/CAS No. | Molecular Formula | Articles |
|---|---|---|---|
 |
Citrate lyase
CAS:9012-83-3 |
|
A novel ATP-generating machinery to counter nitrosative stre...
2015-01-01 [Biochim. Biophys. Acta 1850(1) , 43-50, (2015)] |
|
Acid-inducible transcription of the operon encoding the citr...
2004-09-01 [J. Bacteriol. 186(17) , 5649-60, (2004)] |
|
Identification of the active site of phosphoribosyl-dephosph...
2000-10-31 [Biochemistry 39(43) , 13233-40, (2000)] |
|
Biosynthesis of the prosthetic group of citrate lyase.
2000-08-08 [Biochemistry 39(31) , 9438-50, (2000)] |
|
Identification of a gene cluster in Klebsiella pneumoniae wh...
2002-05-01 [J. Bacteriol. 184(9) , 2439-46, (2002)] |