Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire 1985-07-01

Activation of folypolyglutamate synthetase by pteroic acid.

P J Vickers, R Di Cecco, Z B Pristupa, K G Scrimgeour

Index: Can. J. Biochem. Cell Biol. 63(7) , 777-9, (1985)

Full Text: HTML

Abstract

The glutamylation of methotrexate catalyzed by beef liver folypolyglutamate synthetase (FPGS) is activated by addition of pteroic acid. Pteroic acid causes greater stimulation of FPGS, including glutamylation of tetrahydrofolate, at neutral pH values (i.e., below the pH optimum of 8.4). We have attributed this activation to a conformational change of FPGS induced by pteroic acid.