Biotechnology Letters 2011-03-01

Enzymatic synthesis of sucrose octaacetate using a novel alkaline protease.

Guiying Li, Yujie Cai, Xiangru Liao, Jing Yin

Index: Biotechnol. Lett. 33(3) , 607-10, (2011)

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Abstract

Acylation of 0.5 g sucrose with 1.2 ml acetic anhydride was carried out in 2 ml two-solvent medium of anhydrous pyridine/n-hexane (1:1, v/v) using 0.2 g crude protease from Serratia sp. Sucrose octaacetate was the sole product and more than 90% sucrose was converted in 24 h at 30°C. The purity of sucrose octaacetate reached 100%, via a simple purification method of alcohol/water washing and centrifugation.