Journal of Pharmaceutical Sciences 1984-10-01

Spectrofluorometric determination of captopril plus captopril disulfide metabolites in plasma.

E Ivashkiv

Index: J. Pharm. Sci. 73(10) , 1427-30, (1984)

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Abstract

Captopril disulfides and the drug covalently bound to proteins were reduced with tri-n-butylphosphine. After sample purification on an XAD-2 column, captopril was treated with 1-(7-dimethylamino)-4-methyl-2-oxo-2H -1-benzopyran-3-yl)-1H-pyrrole-2,5-dione to form a fluorescent derivative. After acidification, the fluorescent derivative was extracted into toluene and purified on a C18 cartridge. The fluorescence of the dimethyl-formamide eluate was measured at an excitation wavelength of 380 nm and a fluorescence wavelength of 440 nm.