Steroids 1984-07-01

Determination of 3 beta-hydroxy-5-cholen-24-oic acid and its sulfate in human serum by gas chromatography-mass spectrometry.

M Tohma, H Wajima, R Mahara, T Kurosawa, I Makino, Masahiko Tohma, Hiromi Wajima, Reijiro Mahara, Takao Kurosawa, Isao Makino

Index: Steroids 44(1) , 47-55, (1984)

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Abstract

The glycine conjugate of 3 beta-hydroxy-5-cholen-24-oic acid and its sulfate labeled with deuterium at the C-2, -4, and -23 positions were synthesized. A highly sensitive and specific quantitative assay of the bile acid has been developed by selected ion monitoring in gas chromatography-mass spectrometry of the methyl ester trimethylsilyl ether derivatives using the deuterium labeled conjugates as internal standards. Calibration curves for the bile acid and its sulfate exhibited a linear relationship over the range of 0.01-100 micrograms/ml in human serum.