Journal of chromatography. B, Biomedical applications 1994-05-13

Determination of rhoifolin and daidzin in human plasma by high-performance liquid chromatography.

K Ishii, S Urano, T Furuta, Y Kasuya

Index: J. Chromatogr. B, Biomed. Appl. 655(2) , 300-4, (1994)

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Abstract

A method for determining flavonoids in human plasma is presented for application to pharmacokinetic studies of two flavonoids, rhoifolin and daidzin. Isocratic reversed-phase high-performance liquid chromatography (HPLC) was used with genistin as an internal standard and solid-phase extraction using a Sep-Pak C18 cartridge. The mobile phases were acetonitrile-0.1 M ammonium acetate solution (20:80, v/v) for rhoifolin and methanol-0.1 M ammonium acetate solution (33:67, v/v) for daidzin. The detection limits on-column were 2 ng for rhoifolin and 0.5 ng for daidzin.