Journal of Agricultural and Food Chemistry 2002-08-14

Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays.

Achim Freisleben, Peter Schieberle, Michael Rychlik

Index: J. Agric. Food Chem. 50(17) , 4760-8, (2002)

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Abstract

[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.