Analytical Biochemistry 2011-04-15

Use of dynamic light scattering to determine second virial coefficient in a semidilute concentration regime.

Sandeep Yadav, Thomas M Scherer, Steven J Shire, Devendra S Kalonia

Index: Anal. Biochem. 411(2) , 292-6, (2011)

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Abstract

The present work discusses an alternative procedure to obtain static light scattering (SLS) parameters in a dilute and semidilute concentration regime from a dynamic light scattering (DLS) instrument that uses an avalanche photodiode (APD) for recording the scattered intensity signal. An APD enables one to perform both SLS and DLS measurements by photon counting and photon correlation, respectively. However, due to the associated recovery time, the APDs are susceptible to saturation (above 1000 kcps), which may limit the measurements in systems that scatter too much light. We propose an alternative way of obtaining the SLS parameters with instruments that use APD for recording signal intensities.Copyright © 2010 Elsevier Inc. All rights reserved.