Drug and Chemical Toxicology 1987-01-01

Nucleic acid content and residue determination in tissues of chicks born from 2,4-dichlorophenoxyacetic butyl ester treated eggs.

R O Duffard, A I Fabra de Peretti, S M Castro de Cantarini, G B Mori de Moro, J M Argüello, A M Evangelista de Duffard

Index: Drug Chem. Toxicol. 10(3-4) , 339-55, (1987)

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Abstract

The effects of 2,4-dichlorophenoxyacetic butyl ester (2,4-D b.e.) on protein and nucleic acid contents of different tissues of chicks born from externally treated hen's eggs were studied. Residues of 2,4-D b.e. were also determined and quantified by gas liquid chromatography (GLC). A diminished DNA content was observed in skeletal muscle and stomach, an increased one was found in cerebrum and cerebellum, whereas no variations were observed in the other analysed tissues. Protein levels were found decreased in kidney, cerebrum and heart. Besides, the GLC determinations revealed the presence of the 2,4-D b.e. in all the studied tissues finding the highest concentrations in kidney. 2,4-D did not appear to be a significant metabolite of 2,4-D b.e. in this experimental system. The dissimilar effects of 2,4-D b.e. on protein and nucleic acid contents of the studied tissues would be related to a different tissue susceptibility to the drug and/or to drug distribution.