Journal of Molecular Recognition 2013-04-01

Chiral discrimination and interaction mechanism between enantiomers and serum albumins.

ZhiLi Fang, WenCui Su, WeiGuang Zhang, Yan Xu, YaJin Xiong, MiaoLi Luo, Jun Fan, Xia Yin

Index: J. Mol. Recognit. 26(4) , 161-4, (2013)

Full Text: HTML

Abstract

The chiral discrimination studies of biological system are theoretically and practically significant for the development of chiral drugs and life science. Our work has embarked upon the interaction between serum albumin (SA) (including human SA and bovine SA), R,S-1-(4-methoxyphenyl)ethylamine, and R,S-1-(3-methoxyphenyl)ethylamine. The formation of intermediate transition state, binding sites, and chiral discrimination ability can be investigated by ultraviolet-visible spectra and fluorescence spectra. Moreover, both the changes of hydrophobic microenvironment and energy transfer can be detected by synchronous fluorescence spectra and fluorescence lifetime.Copyright © 2013 John Wiley & Sons, Ltd.