Biochemical Journal 1980-06-01

The isolation of human-erythrocyte band-3 polypeptide labelled with a photosensitive hydrophobic probe.

E Wells, J B Findlay

Index: Biochem. J. 187(3) , 719-25, (1980)

Full Text: HTML

Abstract

To investigate the intramembranous domains of the major band-3 polypeptide, human erythrocyte membranes were labelled with 1-azido-4-[125I]iodobenzene. The anion-exchange protein has been isolated by a new procedure that decreases possible contamination by other integral membrane proteins of similar molecular weight. After chemical modification, the protein was subjected to proteolytic cleavage by several proteinases to yield a distinct pattern of peptides, a few of which contained radioactivity.