Electrophoresis 2011-04-01

Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection.

Worapan Pormsila, Réjane Morand, Stephan Krähenbühl, Peter C Hauser

Index: Electrophoresis 32 , 884-889, (2011)

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Abstract

The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2-morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL-histidine, 70 μmol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10-1000 μmol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 μmol/L. Intra- and inter-day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.