Biochemistry international 1992-05-01

Effects of ethanol and its fluorinated analogues on the calcium ATPase of sarcoplasmic reticulum.

F C Bennett, S D Gates, G F King, B C Shanley, R Smith

Index: Biochem. Int. 26(6) , 979-85, (1992)

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Abstract

The effects of ethanol, monofluoro- and trifluoroethanol on the hydrolysis of ATP and coupled Ca2+ translocation by sarcoplasmic reticulum Ca(2+)-ATPase (EC 3.6.1.38) were measured. All three alcohols had parallel effects on the enzyme activities at concentrations up to 200 mM, suggesting a similar mechanism of action. 19F nuclear magnetic resonance spectroscopy was used to investigate their site of action in the purified enzyme.