Bioscience, Biotechnology, and Biochemistry 2001-01-01

Characterization of PBZ1, a probenazole-inducible gene, in suspension-cultured rice cells.

H Nakashita, K Yoshioka, M Takayama, R Kuga, N Midoh, R Usami, K Horikoshi, K Yoneyama, I Yamaguchi

Index: Biosci. Biotechnol. Biochem. 65(1) , 205-8, (2001)

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Abstract

Probenazole (PBZ) induces non-race specific resistance in rice plants against rice blast fungus and PBZ1 was identified as a PBZ-inducible gene from rice. The induction of PBZ1 expression in suspension-cultured rice cells was investigated. Northern blot analysis indicated that PBZ1 was induced by PBZ in a dose-dependent manner. Enzyme-linked immunosorbent assay (ELISA) showed a dose and time-dependent accumulation of PBZ1 protein. Both mRNA and protein analysis showed that PBZ1 was not induced by salicylic acid or an active metabolite, 1,2-benzisothiazole-1,1-dioxide.