Cellular Immunology 2012-01-01

Overlapping and distinct roles of GRK5 in TLR2-, and TLR3-induced inflammatory response in vivo.

Nandakumar Packiriswamy, Sitaram Parvataneni, Narayanan Parameswaran

Index: Cell. Immunol. 272(2) , 107-11, (2011)

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Abstract

G-protein coupled receptor kinase-5 (GRK5) is a recently described NFκB regulator in TLR4 signaling pathway. To determine whether the role of GRK5 is MyD88- or TRIF-dependent, we injected wild type and GRK5 knockout mice with Pam3CSK4 (MyD88-dependent TLR1/2 ligand) and Poly(I:C) (TRIF-dependent TLR3 ligand) and examined the in vivo systemic inflammatory response. Our results demonstrate that GRK5 regulates IL-12p40 and G-CSF via a mechanism that is common to both MyD88 and TRIF. However, GRK5 regulates IL-5 and MCP-1 in a MyD88-dependent but TNFα in a TRIF-dependent manner. Together, our results demonstrate multiple roles of GRK5 in TLR signaling.Copyright © 2011 Elsevier Inc. All rights reserved.