Chelation as a driving force in synthesis. A new route to α-nitro acids and α-amino acids

M Stiles, HL Finkbeiner

Index: Stiles; Finkbeiner Journal of the American Chemical Society, 1959 , vol. 81, p. 505

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Citation Number: 71

Abstract

Extracts of Escherichia coli contain an enzyme system which catalyzes an oxidative decarboxylation of pyruvate represented by reaction 1. 1, 2-3 acetyl CoA+ COZ+ DPNH+ H+(1) We have obtained highly purified preparations (250-fold purification) of this system from extracts of the Crookes strain. It is apparently an enzyme~ omplex,~ and sediments in the ultracentrifuge (1 to 2 hr. at 144,000 X g) as a dark yellow, fluorescent pellet. The ...