Biochemical and Biophysical Research Communications 1990-08-31

Hydrolysis of synthetic chromogenic substrates by HIV-1 and HIV-2 proteinases.

L H Phylip, A D Richards, J Kay, J Kovalinka, P Strop, I Blaha, J Velek, V Kostka, A J Ritchie, A V Broadhurst

Index: Biochem. Biophys. Res. Commun. 171 , 439, (1990)

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Abstract

Kinetic constants (Km,Kcat) are derived for the hydrolysis of a number of chromogenic peptide substrates by the aspartic proteinase from HIV-2. The effect of systematic replacement of the P2 residue on substrate hydrolysis by HIV-1 and HIV-2 proteinases is examined.