Journal of Chromatography A 1997-08-15

Study of correlations between fine structure of bonded layer and affinity properties of silicas with attached cyclic oligopeptides.

P G Mingalyov, N V Orishchenko, Fadeev AYu

Index: J. Chromatogr. A. 777(2) , 249-59, (1997)

Full Text: HTML

Abstract

The cyclic oligopeptides bacitracin A and gramicidin-S were immobilized on epoxy-, tosyloxy- and halogenoaklyl-activated silicas. The sorbents obtained were tested for affinity binding of proteinases. The effects of spacer length, ligand loading and the character of ligand attachment (one- or poly-site) on the biospecific characteristics have been studied.