Annals of Clinical Biochemistry 1984-09-01

Determination of HDL-cholesterol using 2,4,6-tribromo-3-hydroxybenzoic acid with a commercial CHOD-PAP reagent.

P Trinder, D Webster

Index: Ann. Clin. Biochem. 21 ( Pt 5) , 430-3, (1984)

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Abstract

The Boehringer one-component high-performance cholesterol oxidase reagent has been modified by the inclusion of 2,4,6-tribromo-3-hydroxybenzoic acid (TBHBA) to give a fourfold increase in sensitivity to a molar absorbance of approximately equal to 29,000 with respect to cholesterol. The resulting reagent system is particularly suitable for the determination of plasma HDL-cholesterol for which a reagent of high sensitivity is required. A simple method of bromination avoiding the use of elemental bromine is used to prepare TBHBA. The modified reagent system has been found to have good within- and between-batch precision and has shown itself to be reliable and trouble-free.