Journal of Photochemistry and Photobiology B: Biology 1990-01-01

Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies.

M Síp, P Herman, J Plásek, V Hrouda

Index: J. Photochem. Photobiol. B, Biol. 4 , 321, (1990)

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Abstract

The mechanism of carbocyanine dye diS-C3-(5) fluorescence intensity variations with transmembrane potential changes has been studied using time-resolved fluorescence spectroscopy. Clear evidence is given of the transmembrane-potential-dependent partition of the dye among various sites with different fluorescence lifetimes. It was found that fluorescence decay profiles reflect the transmembrane potential changes.