Analytical Biochemistry 1995-09-20

Halophilic protein stabilization by the mild solubilizing agents nondetergent sulfobetaines.

L Vuillard, D Madern, B Franzetti, T Rabilloud

Index: Anal. Biochem. 230 , 290-4, (1995)

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Abstract

In this work, experiments performed on pig heart and halophilic malate dehydrogenase as well as halophilic elongation factor Tu demonstrate a protein stabilization property from the recently described mild solubilizing agents nondetergent sulfobetaines. A practical application is given by the separation of halophilic bacteria elongation factor Tu and halophilic malate dehydrogenase by high-performance ion-exchange chromatography achieved at reduced salt levels without significant loss of activity.