Bioscience, Biotechnology, and Biochemistry 2011-01-01

Selective, high conversion of D-glucose to 5-keto-D-gluoconate by Gluconobacter suboxydans.

Yoshitaka Ano, Emiko Shinagawa, Osao Adachi, Hirohide Toyama, Toshiharu Yakushi, Kazunobu Matsushita

Index: Biosci. Biotechnol. Biochem. 75(3) , 586-9, (2011)

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Abstract

Selective, high-yield production of 5-keto-D-gluconate (5KGA) from D-glucose by Gluconobacter was achieved without genetic modification. 5KGA production by Gluconobacter suffers byproduct formation of 2-keto-D-gluconate (2KGA). By controlling the medium pH strictly in a range of pH 3.5-4.0, 5KGA was accumulated with 87% conversion yield from D-glucose. The pH dependency of 5KGA formation appeared to be related to that of gluconate oxidizing activity.