Annales of National Institute of Hygiene/Roczniki Panstwowego Zakladu Higieny 2009-01-01

[Determination of azaperone and carazolol residues in animals kidney using LC-MS/MS method].

Iwona Zawadzka, Lech Rodziewicz

Index: Rocz. Panstw. Zakl. Hig. 60(1) , 19-23, (2009)

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Abstract

The method is presented to analyze azaperone and carazolol in pigs kidney. Samples were extracted with acetonitryle. The determination was performed by LC-ESI-MS/MS. The LC was equipped with column Luna C18 Phenomenex. Haloperidol was used as internal standards. The method was validation according to the criteria of Decision Commission No 2002/657/EC. Recoveries for the level 100 microg/kg azaperone and 25 microg/kg carazolol were in the range 91.2-107.0% and 70.8-93.2%. The limit of decision (CCalpha) and detection capability (CCbeta) was respectively 125.9 microg/kg, 160.0 microg/kg for azaperone and 29.3 microg/kg, 33.3 microg/kg. LC-MS/MS technique fulfill the requirements of Decision Commission No 2002/657/EC.