Methods 2012-11-01

ChIA-PET analysis of transcriptional chromatin interactions.

Jingyao Zhang, Huay Mei Poh, Su Qin Peh, Yee Yen Sia, Guoliang Li, Fabianus Hendriyan Mulawadi, Yufen Goh, Melissa J Fullwood, Wing-Kin Sung, Xiaoan Ruan, Yijun Ruan

Index: Methods 58(3) , 289-99, (2012)

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Abstract

Long-range chromatin contacts between specific DNA regulatory elements play a pivotal role in gene expression regulation, and a global characterization of these interactions in the 3-dimensional (3D) chromatin structure is imperative in understanding signaling networks and cell states. Chromatin Interaction Analysis using Paired-End Tag sequencing (ChIA-PET) is a method which converts functional chromatin structure into millions of short tag sequences. Combining Chromatin Immunoprecipitation (ChIP), proximity ligation and high-throughput sequencing, ChIA-PET provides a global and unbiased interrogation of higher-order chromatin structures associated with specific protein factors. Here, we describe the detailed procedures of the ChIA-PET methodology, unraveling transcription-associated chromatin contacts in a model human cell line.Copyright © 2012 Elsevier Inc. All rights reserved.