Journal of chromatography. A 2010-06-25

Optimal determination of steric mass action model parameters for beta-lactoglobulin using static batch experiments.

Tilman Barz, Verena Löffler, Harvey Arellano-Garcia, Günter Wozny

Index: J. Chromatogr. A. 1217 , 4267-77, (2010)

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Abstract

In this work, parameters of the steric mass-formalism SMA are optimally ascertained for a reliable determination of the adsorption isotherms of beta-lactoglobulin A and B under non-isocratic conditions. For this purpose, static batch experiments are used in contrast to the protocols based on different experimental steps, which use a chromatographic column. It is shown that parameters can already be determined for a small number of experiments by using a systematic procedure based on optimal model-based experimental design and an efficient NLP-solver. The in different works observed anti-Langmuir shape of the isotherm for small concentrations of beta-lactoglobulin A was corroborated. Moreover, we also found indications for a porosity variation with changing protein concentrations.Copyright 2010 Elsevier B.V. All rights reserved.