China Journal of Chinese Materia Medica 2013-07-01

[Analysis on component difference in Citrus reticulata before and after being processed with salt by UPLC-Q-TOF/MS].

Rui Zeng, Juan Fu, La-Bin Wu, Lin-Fang Huang

Index: Zhongguo Zhong Yao Za Zhi 38(14) , 2318-20, (2013)

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Abstract

To analyze components of Citrus reticulata and salt-processed C. reticulata by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and compared the changes in components before and after being processed with salt. Principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA) were adopted to analyze the difference in fingerprint between crude and processed C. reticulata, showing increased content of eriocitrin, limonin, nomilin and obacunone increase in salt-processed C. reticulata. Potential chemical markers were identified as limonin, obacunone and nomilin, which could be used for distinguishing index components of crude and processed C. reticulata.