Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2012-09-18

Development of a repeated-dose liver micronucleus assay using adult rats: An investigation of diethylnitrosamine and 2,4-diaminotoluene

Kazunori Narumi, Koji Ashizawa, Rie Takashima, Hironao Takasawa, Seiichi Katayama, Yasuhiro Tsuzuki, Hideki Tatemoto, Takeshi Morita, Makoto Hayashi, Shuichi Hamada

Index: Mutat. Res. 747(2) , 234-9, (2012)

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Abstract

Highlights ► We developed a repeated-dose liver micronucleus (MN) assay using normal adult rats. ► Chemical hepatocarcinogens significantly induced micronucleated hepatocytes in the assay. ► A new hepatocyte isolation technique without in situ perfusion was simultaneously established. ► The assay method enables integration of liver MN assay into general toxicity studies.