Veterinary Parasitology 2013-09-01

Efficacy of decoquinate against Sarcocystis neurona in cell cultures.

David S Lindsay, M Mudasser Nazir, Azhar Maqbool, Siobhan P Ellison, Jeannine S Strobl

Index: Vet. Parasitol. 196(1-2) , 21-3, (2013)

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Abstract

Decoquinate is a quinolone anticoccidial approved for use in the prevention of intestinal coccidiosis in farm animals. This compound has good activity against Toxoplasma gondii and Neospora caninum in cell cultures. The drug acts on the parasites' mitochondria. The activity of decoquinate against developing merozoites of 2 isolates of Sarcocystis neurona was examined in cell culture. Merozoite production at 10 days was completely inhibited when decoquinate was used at 20 or 240 nM. The IC50 of decoquinate was 0.5 ± 0.09nM for the Sn6 isolate of S. neurona from a horse and 1.1 ± 0.6 nM for the SnOP15 isolate of S. neurona from an opossum. Levamisole was toxic at 5 μg/ml and no synergism was observed when decoquinate was combined with levamisole and tested against the Sn3YFP isolate of S. neurona. Decoquinate was cidal for developing schizonts of S. neurona at 240 nM.Copyright © 2013 Elsevier B.V. All rights reserved.