A series of 5, 6-dihydroxypyrimidine analogs were synthesized and evaluated for their anti- HIV activity in vitro. Among all of the analogs, several compounds exhibited significant anti- HIV activity, especially 1b and 1e, which showed the most potent anti-HIV activity with EC50 values of 0.14 and 0.15 μM, and TI (therapeutic index) values of> 300 and> 900, respectively. Further docking studies revealed that the representative compounds 1e and ...